[Complications of mesh-vaginopexy: results of a multicenter trial].

A retrospective multicenter trial with participation of 7 large clinics of Moscow and Moscow Region has been performed to characterize complications after correction of genital prolapse with application of the PROLIFT system. The trial enrolled women with uterine and vaginal prolapse of stage II-IV by POP-Q classification. The women also had anatomic and functional defects of the adjacent organs.

[Peptidylglycine alpha-amidating monooxygenase from bovine heart atrium secretory granules and adrenal chromaffin granules].

About 40-60% of the peptidylglycine alpha-amidating amonooxygenase activity in the lysates of secretory granules from bovine atria and adrenal medulla isolated and lyzed in the presence of pepstatin, phenylmethylsulfonyl gluoride, N-ethylmaleimide and catalase, was found to be in the soluble form. The remaining part bound to the membrane fraction was extracted with Triton X-100. The procedure of purification of the soluble form of peptidylglycine alpha-amidating monooxygenase from both atrial and chromaffin granules in electrophoretically homogeneous enzyme preparations was developed.

[Electron microscopic-cytochemical study of the effect of vinblastine on synaptosome adenylate cyclase in the rat cerebral cortex].

Adenylate cyclase activity was demonstrated by means of electron microscopic cytochemistry in rat cortical synaptosomes incubated under various conditions. It was found that vinblastine caused remarkable changes in the reaction product localization: the limiting membrane reaction diminished, but the number of synaptosomes were seen to contain a course granular precipitate filling up almost the whole presynaptic cytoplasm. The regulatory role the microtubules play in the distribution of adenylate cyclase on cellular membrane is discussed.

Two pools of electron cytochemically demonstrable adenylate cyclase activity in cortical synaptosomes.

Adenylate cyclase activity was demonstrated by means of electron microscopic cytochemistry in rat cortical synaptosomes incubated under various conditions. It was found that only the reaction at the postsynaptic density could be enhanced by noradrenaline and postmortem storage, while that on the presynaptic limiting membrane was insensitive to these effects but was affected selectively by vinblastine. On these grounds two different pools of cytochemically demonstrable adenylate cyclase activity were distinguished within the synaptic region.

Electron microscopic demonstration of adenylate cyclase activity in rat cortical synaptosomes.

The electron cytochemical demonstration of adenylate cyclase activity was carried out in rat cortical synaptosomes. Reaction product was found in 60-70% of the synaptosomes in three predominant localization: (i) on the postsynaptic density; (ii) on the outer aspect of the synaptosomal membrane; (iii) inside the synaptosome. Results suggest that in addition to postsynaptic localization adenylate cyclase activity is cytochemically demonstrable also at presynaptic sites.