In vivo effects of disulfiram and cyanamide on canine liver aldehyde dehydrogenase isoenzymes as detected by high-performance (pressure) liquid chromatography.

Methods for analysis of aldehyde dehydrogenase isoenzymes using high-performance (pressure) liquid chromatography (HPLC) were used to determine in vivo effects of disulfiram and cyanamide on canine liver aldehyde dehydrogenase (ALDH) isoenzymes. Liver ALDH isoenzymes from control and disulfiram- or cyanamide-treated dogs were separated by ion-exchange HPLC, and enzyme activity was detected using a postcolumn reactor. Two major peaks of ALDH activity (peaks I and II) were detected.

Effect of disulfiram on canine liver aldehyde dehydrogenase activity: in vivo inactivation in a nonrodent animal model.

Dogs were used as nonrodent animal models to study the in vivo effects of disulfiram on hepatic aldehyde dehydrogenase (ALDH) activity. Dogs were treated with disulfiram either intraperitoneally or orally (100 mg/kg/day for 2 days followed by 40 mg/kg/day for 3 days). Liver biopsies from control and treated animals were fractionated by differential centrifugation and the subcellular fractions were analyzed for ALDH activity.

Analysis of liver aldehyde dehydrogenase isoenzymes using high-performance (pressure) liquid chromatography.

Methods for separation of isoenzymes using high-performance (pressure) liquid chromatography have been adapted for analysis of aldehyde dehydrogenase (ALDH) (aldehyde:NAD oxidoreductase; EC 1.2.1.