infection model for using human lung tissue.

Introduction: Tularemia is mainly caused by () subsp. () and subsp. () in humans and in more than 200 animal species including rabbits and hares.

Phenotypic and genotypic discrimination of Francisella tularensis ssp. holarctica clades.

Francisella tularensis is the causative agent of tularemia, a zoonotic disease with a wide host range. F. tularensis ssp.

Metabolic adaption of Legionella pneumophila during intracellular growth in Acanthamoeba castellanii.

The metabolism of Legionella pneumophila strain Paris was elucidated during different time intervals of growth within its natural host Acanthamoeba castellanii. For this purpose, the amoebae were supplied after bacterial infection (t =0 h) with 11 mM [U-C]glucose or 3 mM [U-C]serine, respectively, during 0-17 h, 17-25 h, or 25-27 h of incubation. At the end of these time intervals, bacterial and amoebal fractions were separated.

First Description of a Temperate Bacteriophage (vB_KIRK) of Strain 3523.

Here we present the characterization of a bacteriophage (vB__KIRK) including the morphology, the genome sequence and the induction of the prophage. The prophage sequence (FhaGI-1) has previously been identified in strain 3523. UV radiation induced the prophage to assemble phage particles consisting of an icosahedral head (~52 nm in diameter), a tail of up to 97 nm in length and a mean width of 9 nm.