Publications by authors named "K R van Deventer"
Article Synopsis
- Kisspeptin-10 is a hormone that can increase levels of key sex hormones, suggesting its potential use in treating infertility but also posing risks of misuse in sports, leading to its inclusion in the 2024 World Anti-Doping Agency Prohibited List.
- Researchers developed a screening and confirmation method for detecting kisspeptin-10 in urine using liquid chromatography-mass spectrometry, and studied its metabolism in human serum to enhance testing sensitivity.
- The study identified several metabolites of kisspeptin-10 and highlighted a degradation issue possibly caused by oxidation, indicating the need for further research to improve the stability of the substance.
Article Synopsis
- Oncology is shifting towards genome-driven practices, but most cancer diagnostics still rely on traditional microscopy methods, creating delays in using new genomic biomarkers for patient treatment.* -
- Whole-genome sequencing (WGS) can improve diagnostics by detecting numerous genomic changes in one cost-effective test, and it has been shown to be feasible and valid in a clinical setting, with results provided in about 11 workdays.* -
- Successful WGS implementation, such as at the Netherlands Cancer Institute, requires a detailed protocol addressing sample handling and integration into clinical workflows to overcome challenges faced by pathology labs using conventional sample methods.*
Background: The comparative effectiveness of the specific antidote andexanet alfa vs the nonspecific therapy four-factor prothrombin complex concentrates (4F-PCCs) as reversal agents for direct factor Xa (FXa) inhibitors in severely bleeding patients is unclear. We hypothesised that specific reversal using andexanet alfa would be more effective than a high dose of PCC (50 IU kg) for reversing the FXa inhibitor rivaroxaban.
Methods: The reversal potential of andexanet alfa, various 4F-PCCs, and activated PCC was investigated ex vivo in human blood anticoagulated with rivaroxaban (37.
This work presents an ultrafiltration-based, validated method for the screening and confirmation of prohibited growth hormone-releasing hormone (GHRH) analogues (sermorelin/CJC-1293, sermorelin metabolite, CJC-1295 and tesamorelin) in urine by nanoLC-HRMS/MS. Sample preparation avoids the use of laborious antibody-based extraction approaches and consists solely of preconcentration by ultrafiltration. Even in the absence of immuno-affinity purification steps, high sensitivity was still ensured as limits of detection between 5 and 25 pg/mL and limits of identification between 25 and 50 pg/mL were established.
View Article and Find Full Text PDFCombining direct urinary injection with automated filtration and nanoflow LC-MS for the confirmatory analysis of doping-relevant small peptide hormones.
J Chromatogr B Analyt Technol Biomed Life Sci
August 2021
Nano-liquid chromatography (nanoLC) has proven itself as a powerful tool and its scope entails various applications in (bio)analytical fields. Operation at low (nL/min) flow rates in combination with reduced inner dimensions (ID < 100 µm), leads to significantly enhanced sensitivity when coupled with electrospray ionization-mass spectrometry (ESI-MS). Challenges that remain for the routine implementation of such miniaturized setups are related to clogging of the system and robustness in general, and thus the application of tedious sample preparation steps.
View Article and Find Full Text PDF