Antiproliferative, molecular docking, and bioavailability studies of diarylheptanoids isolated from stem bark of Rox B.

Diarylheptanoids are a major class of plant secondary metabolites characterized by 1, 7-diphenyl heptanes in a seven-member carbon frame. In the present study, diarylheptanoids (garuganins 1, 3, 4 and 5) isolated from stem bark were evaluated for cytotoxic activity against MCF-7 and HCT15 cancer cell lines. Among the tested compounds, garuganin and exhibited the highest cytotoxic activity against HCT15 and MCF-7 with IC 2.

Activity Determination of Glycosyltransferases.

The unique chemistry of 2-aminobenzoic acid (2-AA, anthranilic acid, AA) for labeling glycans in aqueous buffer solutions was crucial in developing the assays for measuring the activity of transferases (Anumula, Anal Biochem 457:31-37, 2014). N-acetylglucosamine and N-acetyllactosamine were used as acceptors, and UDP-galactose and CMP-N-acetylneuraminic acid as donors for measuring the activity of β1-4 galactosyltransferases (GalT-1) and α2-6 sialyltransferase (ST-6), respectively. Products formed were labeled in situ with 2-AA and separated from the substrates on a normal-phase TSKgel Amide 80 column.

Analysis of Ser/Thr-Linked Sugar Chains.

Analysis of carbohydrate structures is an integral part of understanding the structure-function relationship of glycans as well as whole glycoproteins. Glycan profiling by HPLC with fluorescence detection is a powerful technique that sheds considerable light into understanding glycan structures. Profiling of N-linked glycans by HPLC and mass spectrometry is well established.

Single tag for total carbohydrate analysis.

Anthranilic acid (2-aminobenzoic acid, 2-AA) has the remarkable property of reacting rapidly with every type of reducing carbohydrate. Reactivity of 2-AA with carbohydrates in aqueous solutions surpasses all other tags reported to date. This unique capability is attributed to the strategically located -COOH which accelerates Schiff base formation.

Quantitative glycan profiling of normal human plasma derived immunoglobulin and its fragments Fab and Fc.

Typical clinical grade human IgG (intravenous immunoglobulin, IVIG), used for carbohydrate analysis, is derived from thousands of healthy donors. Quantitative high-resolution glycan profiles of IgG and its Fc-Fab fragments are presented here. Glycan profiles were established following digestions with Fc specific endoglycosidase S and generic PNGase F under denaturing and non-denaturing (native) conditions.