Thyroliberin (TRH) and TRH free acid (TRH-OH) present in milk do not originate from local synthesis in mammary gland.

Unlabelled: Hypothalamic hormones represent a peculiar group of hormones present in milk in surprisingly high concentrations. High levels of these neuropeptides raised the question of their origin. The hypothesis suggesting local synthesis of TRH in the mammary gland was, therefore, tested.

Decrease in intensity of DNA fluorescence caused by interaction between DNA and platinum complexes.

The decrease of DNA fluorescence caused by an impaired capacity of ethidium bromide to intercalate into the DNA reflected structural changes caused in the DNA molecule by its interaction with platinum complexes. This fall in DNA fluorescence was proportional to the length of exposure of DNA to the platinum complexes, and depended on the environment in which the interaction took place. The therapeutically active cisplatinum (cis-DDP) was more efficient to inhibit fluorescence in a solution of 4 X 10(-3) mol NaCl than its therapeutically inactive trans-isomer (trans-DDP).