Optimization of selectivity in high-performance liquid chromatography using desirability functions and mixture designs according to PRISMA.

A computer program for the mobile phase optimization of high-performance liquid chromatography (HPLC) is described. The desirability function technique combined to the prisma mixture design was employed to enhance the quality of HPLC separations. The use of statistical models to predict the behaviour of retention times (tR) and band broadening at the different eluent compositions obtained by prisma was examined for dansyl amides and coumarins.

Behaviour of resolution by changing solvent strength and selectivity in the 'PRISMA' model using reversed-phase HPLC for biogenic amines.

The retention behaviour of 17 dansylated biogenic amines in 6 linear gradients at 13 solvent combinations, expressed by the selectivity points (Ps) according to the 'PRISMA' model, was investigated. The dependence between the retention times (tr) and different gradients was examined. Three dimensional resolution (Rs) maps for each peak-pair in the different gradients at the 13 selectivity points were constructed, and the affect of the gradients on separation were investigated.

Optimization of the HPLC analysis of biogenic amines in Peucedanum palustre plants and cell culture lines.

The HPLC separation of dansylated amides including mono-, di-, and polyamines from a Peucedanum palustre (L.) Moench plant and an embryogenic cell culture line was studied. The flowers, stems, leaves, and roots of the plant were analyzed separately.