Biological activity of des-(B26-B30)-insulinamide and related analogues in rat hepatocyte cultures.

Short-term and long-term biological activities were studied in adult rat hepatocytes cultured in the presence of the insulin analogues des-(B26-B30)-insulinamide, [TyrB25]des-(B26-B30)-insulinamide and [HisB25]des-(B26-B30)-insulinamide. When compared to insulin, full potency of des-(B26-B30)-insulinamide has been reported in rat adipocytes and an enhanced potency has been reported for the other analogues. Steady state binding characteristics of the analogues to hepatocytes were indistinguishable from those of native insulin with half-maximal binding occurring at concentrations of about 0.