Open-label multicenter registry on the outcomes of peripheral arterial disease treated by balloon angioplasty with optical frequency domain imaging in superficial femoral artery and popliteal artery (OCEAN-SFA study).

Although balloon angioplasty for femoropopliteal artery lesions has been associated with restenosis rates of up to 60% at 12 months, the mechanism of restenosis has not been fully evaluated. The aim of this study was to evaluate the relationship between the vascular features observed on optical frequency domain imaging (OFDI) before and after balloon angioplasty of femoropopliteal artery lesions, and restenosis at 6 months. This study was a prospective multicenter single arm study.

Real-time monitoring in three-dimensional hepatocytes reveals that insulin acts as a synchronizer for liver clock.

Resetting the peripheral clock and understanding the integration between the circadian rhythm and metabolic pathways are fundamental questions. To test whether insulin acts as a synchronizer for the hepatic clock by cell-autonomous mechanisms, the phase-resetting capabilities of insulin were investigated in cultured hepatic cells. We provide evidence that three-dimensional (3D) cell culture conditions that preserve the differentiated state of primary hepatocytes sustained the robustness of the molecular clock, while this robustness rapidly dampened under classical monolayer cell culture conditions.

Regular feeding plays an important role in cholesterol homeostasis through the liver circadian clock.

Rationale: Peripheral clock control and the relevance of the circadian rhythm to physiology and disease are major questions in mammalian circadian biology.

Objective: We examined the physiological functions of the liver clock.

Methods And Results: We established a suppressed feeding schedule regimen constituting a high-cholesterol diet delivered every 6 hours without changes in energy and cholesterol intake.

Metabolic Fate of β-Aspartyl-(14)C-glycine in Normal Young Rats.

The metabolic fate of α- and β-L-aspartyl-[U-(14)C]glycine was investigated in normal young rats in vivo and in vitro. The radioactive dipeptides were synthesized from L-aspartic acid and [U-(14)C]glycine in our laboratory. When labeled β-aspartylglycine was given intraperitoneally, about 66% of the dose was excreted in the urine and 8% was recovered in the expired carbon dioxide over a 24-hr period.