Publications by authors named "K Nonomura"
Small RNAs are highly abundant and play important roles in plant reproduction. Profiling of small RNAs in reproductive tissues is a critical step in understanding their biology. Here, we describe a protocol for small RNA profiling in rice anthers, with a focus on an abundantly expressed but little-understood reproductive small RNA class named 24-nucleotide phased secondary small interfering RNAs (24-nt phasiRNAs).
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- The study highlights the increasing importance of medical and nursing services in Japan due to an aging population, with a focus on the need for aseptic preparation services in community pharmacies.
- It found that only 8% of community pharmacies offer these services and identified significant regional disparities using Gini coefficients and geographic information systems.
- The conclusion emphasizes that the current distribution of pharmacies providing aseptic preparation is insufficient, and action is necessary to improve access as the population changes by 2050.
Background And Aims: Fertilization relies on pollen mother cells able to transition from mitosis to meiosis to supply gametes. This process involves remarkable changes at the molecular, cellular and physiological levels, including (but not limited to) remodelling of the cell wall. During the onset of meiosis, the cellulose content in the pollen mother cell walls gradually declines, with the concurrent deposition of the polysaccharide callose in anther locules.
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- In angiosperms, epigenetic profiles crucial for genomic imprinting are set up before fertilization, but the connection between these modifications and gene expression is not fully clear.
- This research categorized imprinted genes in rice endosperm into 'persistent' and 'stage-specific' types based on a transcriptome analysis over time and found differences in the levels of epigenetic modifications between them.
- The study showed that maternal allele activation in maternally expressed imprinted genes is linked to DNA demethylation, while paternally expressed genes with gene body methylation are silenced through similar processes and associated with specific genetic motifs.