Antimicrobial Resistance and Biofilm Formation of Isolated from Pig Farms and Surroundings in Bulgaria.

() is a ubiquitous microorganism with pathogenic and saprophytic clones. The objective of this study was to evaluate the presence, virulence, antibiotic resistance and biofilm formation of in three industrial farms in Bulgaria, as well as their adjacent sites related to the utilization of manure (feces, wastewater in a separator, lagoons, means of transport, and soils). The isolation of single bacterial cultures was performed via standard procedures with modifications, and isolates were identified via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR).

SPECT-CT Imaging with [99mTc]PSMA-T4 in patients with Recurrent Prostate Cancer.

Background: Prostate-specific membrane antigen (PSMA) is a cell surface glycoprotein with a large extracellular domain with overexpression of the prostatic tumour cells. Several small molecules of PSMA ligands of inhibitors binding to the active site of PSMA were developed. [99mTc]Tc-PSMA-T4 is a new radiopharmaceutical (Polatom) for imaging loco-regional metastases and/or local relapse in patients with prostate cancer.

Effect of high concentrations of sucrose on the enzymatic activity of alpha-chymotrypsin.

The effect of the low-molecular-mass natural reagents in high concentrations is important for investigating enzymatic reactions in near "in vivo" conditions and for optimisation of biotechnology processes. A model system, including p-nitrophenyl acetate as substrate and alpha-chymotrypsin as proteolytic enzyme, has been used to study the effect of high concentrations of sucrose, both influencing the viscosity of the reaction medium and acting as a nucleophilic effector (activator) on the enzymatic reaction. A kinetic scheme at high concentrations of nucleophilic effectors (sucrose) has been proposed.

[Epidemiological examinations of the workers at a thermoelectric power station].

Medical and clinico-laboratory examinations were performed of thermoelectric power station (TES) workers who were exposed to the hazardous factors related to the technological process and contamination substances transferred from other industrial sites. Following prepathologic and pathologic states were registered: respiratory functional disorders, ischemic heart disease, arterial hypertension, audiometric state deviations and acoustic nerve neuritis, irritative dermatitis. Basing on the data obtained, risk groups identification was performed.

[Distribution of atomic density in protein globules].

The distribution of atomic density in the elastase and subtilysin globulas was modeled on the basis of their x-ray atomic coordinates and was consequently investigated through comparison with the normal Poisson distribution, using the chi2-test. The results from the above procedure are discussed in connection with the concept of kinetic constraints on the globula folding process as well as with the enzyme-machine hypothesis. It is shown that the minimum average dimensions of atomic irregularities in the protein globula are about 200 A3.