Publications by authors named "K N Mwange"

As part of a worldwide survey of the indoor mycobiota, dust was collected from nine countries. Analyses of dust samples included the culture-dependent dilution-to-extinction method and the culture-independent 454-pyrosequencing. Of the 7 904 isolates, 2 717 isolates were identified as belonging to Aspergillus, Penicillium and Talaromyces.

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Aims: The development and evaluation of a sensitive and specific TaqMan real-time polymerase chain reaction (PCR) for the detection and identification of Pantoea stewartii on maize.

Methods And Results: A TaqMan-based real-time PCR assay targeting the cpsD gene enabling specific detection of P. stewartii in maize leaves and seeds was developed.

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Article Synopsis
  • A cDNA clone of the auxin binding protein 1 (ABP1) from Eucommia ulmoides was isolated to study its seasonal expression in relation to plant hormones IAA and ABA using various molecular techniques.
  • The expression of ABP1 varied throughout the cambial stages, being high during active stages and scarce during resting stages, indicating its involvement in the plant's growth cycle.
  • The findings suggest that IAA enhances ABP1 expression and the reactivation of a quiescent cambium, while ABA inhibits it, highlighting ABP1's role in linking auxin response to cambial activity.
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The seasonal change of free abscisic acid (ABA) and indole-3-acetic acid (IAA) and their relationship with the cambial activity in Eucommia ulmoides trees were investigated by ABA and IAA immunolocalization using primary polyclonal and rhodamine-red fluorescing secondary antibodies, ABA and IAA quantification using high performance liquid chromatography (HPLC), and systematic monitoring of vascular cell layers production. ABA and IAA clearly displayed opposite annual distribution patterns. In the active period (AP), both immunolocalization and HPLC detected an abrupt decrease of ABA, reaching its lowest level in the summer.

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Eucommia ulmoides (Eucommiaceae), a traditional Chinese medicinal plant, is often subjected to severe manual peeling of its bark. If the girdled trunk is well protected from desiccation, new bark forms within 1 month. It has been proposed that phytohormones play a key role in this process.

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