Dynamic interface printing.

Additive manufacturing is an expanding multidisciplinary field encompassing applications including medical devices, aerospace components, microfabrication strategies and artificial organs. Among additive manufacturing approaches, light-based printing technologies, including two-photon polymerization, projection micro stereolithography and volumetric printing, have garnered significant attention due to their speed, resolution or potential applications for biofabrication. Here we introduce dynamic interface printing, a new 3D printing approach that leverages an acoustically modulated, constrained air-liquid boundary to rapidly generate centimetre-scale 3D structures within tens of seconds.

Enhanced acoustic streaming effects sharp-edged 3D microstructures.

Acoustofluidic micromanipulation is an important tool for biomedical research, where acoustic forces offer the ability to manipulate fluids, cells, and particles in a rapid, biocompatible, and contact-free manner. Of particular interest is the investigation of acoustically driven sharp edges, where high tip velocity magnitudes and strong acoustic potential gradients drive rapid motion. Whereas prior devices utilizing 2D sharp edges have demonstrated promise for micromanipulation activities, taking advantage of 3D structures has the potential to increase their performance and the range of manipulation activities.

Quantitative mechanical stimulation of GPR68 using a novel 96 well flow plugin.

Mechanosensitive proteins play a crucial role in a range of physiological processes, including hearing, tactile sensation and regulating blood flow. While previous work has demonstrated the mechanosensitivity of several proteins, the ability to apply precisely defined mechanical forces to cells in a consistent, replicable manner remains a significant challenge. In this work we present a novel 96-well plate-compatible plugin device for generating highly-controlled flow-based mechanical simulation of cells, which enables quantitative assessment of mechanosensitive protein function.

Thomson-Einstein's Tea Leaf Paradox Revisited: Aggregation in Rings.

A distinct particle focusing spot occurs in the center of a rotating fluid, presenting an apparent paradox given the presence of particle inertia. It is recognized, however, that the presence of a secondary flow with a radial component drives this particle aggregation. In this study, we expand on the examination of this "Thomson-Einstein's tea leaf paradox" phenomenon, where we use a combined experimental and computational approach to investigate particle aggregation dynamics.

Optimizing coupling layer and superstrate thickness in attachable acoustofluidic devices.

Superstrate-based acoustofluidic devices, where the fluidic elements are reversibly coupled to a transducer rather than bonded to it, offer advantages for cost, interchangeability and preventing contamination between samples. A variety of coupling materials can be used to transmit acoustic energies into attachable superstrates, though the dimensions and material composition of the system elements are not typically optimized. This work analyzes these coupling layers for bulk wavefront transmission, including water, ultrasound gel and polydimethylsiloxane (PDMS), as well as the material makeup and thickness of the superstrate component, which is commonly comprised of glass, quartz or silicon.