Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines.

Genetic medicines, including CRISPR/Cas technologies, extend tremendous promise for addressing unmet medical need in inherited retinal disorders and other indications; however, there remain challenges for the development of therapeutics. Herein, we evaluate genome editing by engineered Cas9 ribonucleoproteins (eRNP) in vivo via subretinal administration using mouse and pig animal models. Subretinal administration of adenine base editor and double strand break-inducing Cas9 nuclease eRNPs mediate genome editing in both species.

Can an Algorithm Tell How Spiritual You Are? Using Generative Pretrained Transformers for Sophisticated Forms of Text Analysis.

Objective: Text analysis is a form of psychological assessment that involves converting qualitative information (text) into quantitative data. We tested whether automated text analysis using Generative Pre-trained Transformers (GPTs) can match the "gold standard" of manual text analysis, even when assessing a highly nuanced construct like spirituality.

Method: In Study 1, N = 2199 US undergraduates wrote about their goals (N = 6597 texts) and completed self-reports of spirituality and theoretically related constructs (religiousness and mental health).

Pediatric Triage Accuracy in Pediatric and General Emergency Departments.

Background And Objectives: Accurate triage at the time of emergency department (ED) presentation is critical for timely acuity assessment and anticipating resource requirements. Commonly, triage is conducted using the Emergency Severity Index (ESI); however, the accuracy of this approach for children in general EDs is uncertain. The purpose of this study was to quantify pediatric triage accuracy in a national sample of ED visits and evaluate whether presentation to a pediatric vs general ED is associated with mistriage.

Developing methods to identify resilience and improve communication about diagnosis in pediatric primary care.

Communication underlies every stage of the diagnostic process. The Dialog Study aims to characterize the pediatric diagnostic journey, focusing on communication as a source of resilience, in order to ultimately develop and test the efficacy of a structured patient-centered communication intervention in improving outpatient diagnostic safety. In this manuscript, we will describe protocols, data collection instruments, methods, analytic approaches, and theoretical frameworks to be used in to characterize the patient journey in the Dialog Study.