Publications by authors named "K M Riker"
To ensure the quality, safety and efficacy of medicinal products, it is necessary to develop and execute appropriate manufacturing process and product control strategies. Traditionally, product control strategies have focused on testing known quality attributes with limits derived from levels administered in preclinical and clinical studies with an associated statistical analysis to account for variability. However, not all quality attributes have impact to the patient and those with the potential to impact safety and efficacy may not be significant when dosed at patient-centric levels.
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- In neurodegenerative diseases, the shape and structure of β-sheet amyloids play a crucial role, with their chirality (left- or right-handed) affecting their function and disease onset.
- Researchers have studied the amyloid-β 42 sequence and created variations that allow for switching chirality at relevant body temperatures.
- This chiral inversion not only influences the amyloids but also enhances drug release for anticancer treatments, presenting a new way to modify amyloid shapes for potential therapies in neurodegenerative and other diseases.
The integration of proteins with DNA nanotechnology would enable materials with diverse applications in biology, medicine, and engineering. Here, we describe a method for the incorporation of bioactive fibronectin domain proteins with DNA nanostructures using two orthogonal coiled-coil peptides. One peptide from each coiled-coil pair is attached to a DNA origami cuboid in a multivalent fashion by attaching the peptides to DNA handles.
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- - The native extracellular matrix influences cell behavior by dynamically displaying signals, making it crucial to mimic this environment for studying cell-matrix interactions.
- - Researchers developed a synthetic platform using coiled-coil peptides to control the temporal display of cell-adhesive signals, enabling them to manipulate how ligands interact with cells.
- - By varying the timing and exposure of RGDS ligands, the study found that fibroblast cells could reversibly spread and contract, indicating a stronger response to dynamic signals compared to constant signals, which could help in programming cellular behavior.
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- The arrangement of biomolecules in the extracellular matrix influences how cells behave, making it crucial to engineer biomaterials with precisely organized bioactive signals at the nanoscale.
- This study utilized a composite approach with peptide amphiphile (PA) nanofibers and silica nanoparticles, which can be easily modified to include various bioactive signals.
- By clustering specific bioactive signals on silica nanoparticles, the researchers enhanced fibroblast cell adhesion and spreading in composite hydrogels, demonstrating the effectiveness of using multiple integrin-binding sequences for improved cell behavior.