Publications by authors named "K M O Hanschmann"
Article Synopsis
- The study explores a new method for testing the potency of diphtheria, tetanus, and acellular pertussis (DTaP) vaccines, shifting from traditional in vivo animal testing to simultaneous serological testing on guinea pigs.
- It used established WHO International Standards as references and developed a quantification technique for detecting IgG levels against the vaccine antigens through an advanced immunoassay.
- The results demonstrated the effectiveness of this serological approach in identifying vaccine potency and safety, paving the way for its routine application in place of in vivo animal tests, aligning with ethical considerations pertinent to the 3Rs principle (Replace, Reduce, Refine).
Background: Celery root is known to cause severe allergic reactions in patients sensitized to mugwort pollen.
Objective: We studied clinically well-characterized patients with celery allergy by IgE testing with a comprehensive panel of celery allergens to disentangle the molecular basis of what is known as the celery-mugwort syndrome.
Methods: Patients with suspected food allergy to celery underwent a standardized interview.
Human genetic studies based on the Marburg I polymorphism in the factor VII activating protease (FSAP) encoding gene, analysis of FSAP activity in plasma and biochemical characterization of FSAP substrates indicate a possible causal link between FSAP activity and venous thrombosis. We hypothesized that a direct standardized assay to measure FSAP activity in plasma could provide convincing arguments for or against such a potential link. Using Ac-Pro-DTyr-Lys-Arg-AMC as a highly specific and sensitive substrate, histones as a trigger to activate pro-FSAP and plasma-purified active FSAP as a calibrator, we have developed a fluorogenic kinetic assay that reveals the FSAP generating potential in human plasma in real time.
View Article and Find Full Text PDFBackground: Laboratory diagnosis of Lyme borreliosis refers to some methods with known limitations. Molecular diagnostics using specific nucleic acid probes may overcome some of these limitations.
Methods: We describe the novel reporter fluorescence real-time polymerase chain reaction (PCR) probe system for detection of species.
Background And Objectives: Red blood cell concentrates (RBCC) are susceptible to bacterial contamination despite cold storage. A reliable evaluation of strategies to minimize the risk of RBCC-associated bacterial transmission requires the use of suitable reference bacteria. Already existing Transfusion-Relevant Bacteria Reference Strains (TRBRS) for platelet concentrates fail to grow in RBCC.
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