Publications by authors named "K M Klimina"
Article Synopsis
- The study explores using lytic bacteriophages combined with the antibiotic linezolid to treat methicillin-resistant bacteria, showing a strong synergistic effect against planktonic cells.
- A checkerboard assay indicated that a specific combination of low doses of both agents completely inhibited bacterial growth, but the order of treatments affected biofilm cells—sequential treatment was less effective while simultaneous treatment was more beneficial.
- Transcriptomic analysis revealed that the combination altered bacterial metabolism, including energy and virulence factors, emphasizing the need to optimize treatment strategies for maximum effectiveness against infections.
Background: Multiple sclerosis (MS) is a neuroinflammatory disease triggered by a combination of genetic traits and external factors. Autoimmune nature of MS is proven by the identification of pathogenic T cells, but the role of autoantibody-producing B cells is less clear. A comprehensive understanding of the development of neuroinflammation and the identification of targeted autoantigens are crucial for timely diagnosis and appropriate treatment.
View Article and Find Full Text PDFAntimicrobial resistance poses a serious threat to global public health. The COVID-19 pandemic underscored the need to monitor the dissemination of antimicrobial resistance genes and understand the mechanisms driving this process. In this study, we analyzed changes to the oropharyngeal and fecal resistomes of patients with COVID-19 undergoing therapy in a hospital setting.
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- The study focuses on how gut microbiota, particularly commensal bacteria like lactobacilli, interact with the host immune system's cytokines, which are critical in managing inflammation and gut health.
- Using next-generation RNA sequencing, researchers examined the transcriptional responses of lactobacillus strains K32 and R19-3 to various cytokines, revealing significant changes in gene expression linked to metabolism and stress response, especially upon exposure to IL-8 and IL-10.
- The findings highlight a complex adaptation mechanism where these bacteria adjust their gene expression in response to inflammatory signals, paving the way for potential probiotic therapies for conditions like inflammatory bowel disease (IBD).
In the context of the antimicrobial therapy crisis, the significance of studying and implementing alternative treatment methods, particularly phage therapy, is increasingly evident. This study aimed to investigate the resistance of clinical Staphylococcus aureus ST239 strains to Herelleviridae phages through comparative genomics, transcriptomics, and proteomics. Analysis of resistant and sensitive S.
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