Evaluation of One Step Nucleic Acid Amplification for detection of lymph node metastases compared to histopathological ultrastaging in women with endometrial cancer: a protocol for a diagnostic accuracy study.

Background: This is a multicentre, European, prospective trial evaluating the diagnostic accuracy of One Step Nucleic Acid Amplification (OSNA) compared to sentinel lymph nodes histopathological ultrastaging in endometrial cancer patients.

Methods: Centres with expertise in sentinel lymph node mapping in endometrial cancer patients in Europe will be invited to participate in the study. Participating units will be trained on the correct usage of the OSNA RD-210 analyser and nucleic acid amplification reagent kit LYNOAMP CK19 E for rapid detection of metastatic nodal involvement, based on the cytokeratin 19 (CK19) mRNA detection.

Synthesis of Schiff bases based on Chitosan, thermal stability and evaluation of antimicrobial and antitumor activities.

A Schiff base of Chitosan was prepared by condensing of the Chitosan (CS) with six aromatic aldehydes and confirmed by FT-IR, NMR, XRD, TGA, and DSC. XRD results showed the disappeared of peaks at 2θ = 10° for CS and appeared one peaks at around 2θ of 23° for Schiff bases, while TGA was demonstrated that the thermal stability of CS has improved after the modification with the corresponding aldehyde. Also, DSC shows endothermal peak of CS at 100 °C due to the loss of water and second thermal event related to the decomposition of amine units with an exothermic peak at 295 °C, while Schiff bases shows endothermal peak around 70-95 °C which is related to the loss of water for all samples and the second exothermic peak around 260-280 °C is related to the decomposition of the amine group in the polymer units.

One-Step Nucleic Acid Amplification Analysis of Sentinel Nodes in Endometrial Cancer Versus Ultrastaging: First Long-Term Follow-Up Data of Discordant Cases.

Aims: Endometrial cancer (EC) is the most common gynecological cancer worldwide and its incidence is rising. The cornerstone of its management is surgical treatment with nodal staging. A monocentric study investigating the potential of the molecular biology method of one-step nucleic acid amplification (OSNA) in sentinel lymph node (SLN) analysis was conducted at our institution between April 2016 and January 2018.

Role of the Radical Character in Singlet Fission: An Ab Initio and Quantum Chemical Topology Analysis.

The radical character of molecules exhibiting singlet fission is related to the energy level matching relationships that facilitate this process. Using a linear H model molecule, we employ quantum chemical topology descriptors based on full configuration interaction calculations to rationalize singlet fission. In this context, the influence of the closed-shell to diradical and diradical to tetraradical character on the singlet fission energy matching conditions is analyzed.

Assessing Clinicians' Documentation of Vision in Older Adults Who Presented With a Fall at the Accident and Emergency Department of Northampton General Hospital.

Background Falls among elderly adults are one of the most common reasons that could lead to injury and modality, as vision is one of the modifiable risk factors for falls. By assessing it, we can detect those needing further follow-up with opticians or ophthalmologists, lowering the risk of falls secondary to poor vision. Methods Data were collected and reviewed retrospectively from a consecutive list of patients who presented with a fall or head injury to the Accident and Emergency Department at Northampton General Hospital.