Formative research and development of a referral toolkit for sexual health services linkages within chicago public high schools.

Objective: Linking adolescent students to sexual health services has been recommended to promote overall adolescent health and wellbeing. Support at the school level is needed to develop such linkages, particularly in large, urban districts.

Data Collection Methods: A series of focus groups and interviews were conducted in three high schools piloting sexual health services linkage efforts in Chicago Public Schools.

Enzymatic synthesis of azide by a promiscuous N-nitrosylase.

Azides are energy-rich compounds with diverse representation in a broad range of scientific disciplines, including material science, synthetic chemistry, pharmaceutical science and chemical biology. Despite ubiquitous usage of the azido group, the underlying biosynthetic pathways for its formation remain largely unknown. Here we report the characterization of an enzymatic route for de novo azide construction.

Nursing diagnoses in patients with COVID-19 admitted to the intensive care unit: CROSS-MAPPING.

To identify and analyze the nursing diagnoses listed in the medical records of COVID-19 patients admitted to an intensive care unit using Taxonomy II of NANDA-I. : COVID-19 is a complex disease with heterogeneous behaviors, and the role of intensive care nurses in accurately identifying related signs and symptoms has become even more critical during the pandemic. Nurses rely on classification systems or taxonomies to standardize concepts and language in practice.

Accuracy of the nursing diagnosis of ineffective airway clearance in intensive care unit patients.

Objectives: to analyze the accuracy of the clinical indicators of ineffective airway clearance in adult intensive care unit patients.

Methods: diagnostic accuracy study, performed in the intensive care unit of a university hospital in northeastern Brazil. The sample consisted of 104 patients hospitalized between June and October 2019.

Biosynthesis of Isonitrile Lipopeptide Metallophores from Pathogenic Mycobacteria.

Isonitrile lipopeptides (INLPs) are known to be related to the virulence of pathogenic mycobacteria by mediating metal transport, but their biosynthesis remains obscure. In this work, we use in vitro biochemical assays, site-directed mutagenesis, chemical synthesis, and spectroscopy techniques to scrutinize the activity of core enzymes required for INLP biosynthesis in mycobacteria. Compared to environmental , pathogenic employ a similar chemical logic and enzymatic machinery in INLP biosynthesis, differing mainly in the fatty-acyl chain length, which is controlled by multiple enzymes in the pathway.