Rapid synthesis and screening of natively paired antibodies against influenza hemagglutinin stem via oPool display.

Antibody discovery is crucial for developing therapeutics and vaccines as well as understanding adaptive immunity. However, the lack of approaches to synthesize antibodies with defined sequences in a high-throughput manner represents a major bottleneck in antibody discovery. Here, we presented oPool display, which combines oligo pool synthesis and mRNA display to construct and characterize many natively paired antibodies in parallel.

population dynamics in a vancomycin-induced murine model of gastrointestinal carriage.

is a common nosocomial pathogen and a major cause of morbidity and mortality in hospitalized patients. Multiple reports highlight that gastrointestinal colonization may precede systemic infections by this pathogen. Gaining a deeper insight into the dynamics of gastrointestinal carriage is an essential step in managing gastrointestinal colonization and could contribute to preventing bacterial transmission and progression to systemic infection.

Temporal and spatial dynamics of central nervous system infection in mice.

is a bacterial pathogen that can cause life-threatening central nervous system (CNS) infections. While mechanisms by which and other pathogens traffic to the brain have been studied, a quantitative understanding of the underlying dynamics of colonization and replication within the brain is still lacking. In this study, we used barcoded to quantify the bottlenecks and dissemination patterns that lead to cerebral infection.

Reverse transcriptase inhibitors prevent liver abscess formation during bloodstream infection.

The presence of bacteria in the bloodstream is associated with severe clinical outcomes. In mice, intravenous inoculation of can lead to the formation of macroscopic abscesses in the liver. Abscesses are regions of severe necrosis and consist of millions of bacteria surrounded by inflammatory immune cells.

Genetic and immune determinants of liver abscess formation.

Systemic infections can yield distinct outcomes in different tissues. In mice, intravenous inoculation of leads to bacterial replication within liver abscesses, while other organs such as the spleen clear the pathogen. Abscesses are macroscopic necrotic regions that comprise the vast majority of the bacterial burden in the animal, yet little is known about the processes underlying their formation.