The nucleotide sequence and comparative analysis of the H-2Dp class I H-2 gene.

We present the complete nucleotide sequence and the deduced amino acid sequence of the H-2Dp class I gene. This gene, which was cloned from a B10.P genomic DNA library, encodes and intact, functional H-2Dp molecule.

Detection of H-2 antigens on 8-cell mouse embryos.

H-2 antigens were detected on 80-cell mouse embryos of the b, k, and d haplotypes, but not on 8-cell embryos of the a haplotype. The technique used for detection of H-2 antigens on the embryos was a highly sensitive, automated, complement-dependent cytotoxicity assay, based on the principle that live embryos will incorporate 3H-thymidine into DNA, but embryos killed with antiserum and complement will not. The amount of H-2 present on 8-cell embryos is apparently less than that present on blastocysts.

Specificity of H-2 antigens expressed on mouse blastocysts.

A highly sensitive cytotoxicity assay was used to detect H-2 antigens on mouse blastocyst stage embryos of the b, a, k, and d haplotypes. The assay was based on the principle that live embryos incorporate 3H-thymidine into DNA whereas embryos killed with antiserum and complement do not. The use of specific alloantisera showed that blastocysts of different haplotypes express different H-2 antigens.