Publications by authors named "K Lukyanov"
Post-translational modifications of histones play a crucial role in chromatin structure maintenance and epigenetic regulation. The LiveMIEL (Live-cell Microscopic Imaging of Epigenetic Landscape) method represents a promising approach for tracking histone modifications. It involves visualization of epigenetic modifications using genetically encoded fluorescent sensors and further analysis of the obtained intranuclear patterns by multiparametric image analysis.
View Article and Find Full Text PDFEpigenetic modifications (methylation, acetylation, etc.) of core histones play a key role in regulation of gene expression. Thus, the epigenome changes strongly during various biological processes such as cell differentiation and dedifferentiation.
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- The study focuses on PLpro, a protease in SARS-CoV-2 that helps the virus replicate and evade immunity, and describes a new sensor to detect its activity in live cells.
- The sensor, called PLpro-ERNuc, uses fluorescent proteins to indicate PLpro activity by translocating a green signal to the nucleus when PLpro cleaves a specific site.
- Testing in HeLa cells and SARS-CoV-2-infected Huh7.5 cells showed that the sensor can effectively monitor PLpro activity, highlighting its potential as a tool for screening inhibitors and studying virus dynamics.
Post-translational modifications of histones to a large extent determine the functional state of chromatin loci. Dynamic visualization of histone modifications with genetically encoded fluorescent sensors makes it possible to monitor changes in the epigenetic state of a single living cell. At the same time, the sensors can potentially compete with endogenous factors recognizing these modifications.
View Article and Find Full Text PDFThe real-time monitoring of the intracellular pH in live cells with high precision represents an important methodological challenge. Although genetically encoded fluorescent indicators can be considered as a probe of choice for such measurements, they are hindered mostly by the inability to determine an absolute pH value and/or a narrow dynamic range of the signal, making them inefficient for recording the small pH changes that typically occur within cellular organelles. Here, we study the pH sensitivity of a green-fluorescence-protein (GFP)-based emitter (EGFP-Y145L/S205V) with the alkaline-shifted chromophore's pKa and demonstrate that, in the pH range of 7.
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