Publications by authors named "K Lebani"

Cell culture underpins virus isolation and virus neutralisation tests, which are both gold-standard diagnostic methods for foot-and-mouth disease (FMD). Cell culture is also crucial for the propagation of inactivated foot-and-mouth disease virus (FMDV) vaccines. Both primary cells and cell lines are utilised in FMDV isolation and propagation.

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  • - The study assessed the prevalence of drug resistance mutations (DRMs) to integrase strand transfer inhibitors (INSTIs) just before Botswana switched to dolutegravir (DTG) for first-line HIV treatment in 2016, analyzing over 5,000 HIV-1 sequences from various individuals.
  • - Results indicated that the overall prevalence of significant INSTI DRMs was 1.11%, with higher rates found in those who had not received antiretroviral treatment (ART-naïve individuals) compared to those who had (ART-experienced individuals).
  • - Notable mutations associated with INSTI resistance included E138K and G140R, but high-level resistance to newer drugs like dolutegravir
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  • This study focuses on creating specific primers and a TaqMan probe for the rapid detection and serotyping of the Southern African territory 2 (SAT2) strain of Foot-and-Mouth Disease (FMD).* -
  • The new SAT2 RT-qPCR assay showed high performance, with an efficiency of 99% and favorable comparisons to existing methods like ELISA and VP1 sequencing, particularly demonstrating a strong agreement with VP1 sequencing.* -
  • Future work is needed to develop similar assays for other serotypes (SAT1 and SAT3) and to collect more field samples to validate the effectiveness of the new SAT2 detection method.*
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Certain lineages of the wine, beer and bread yeast Saccharomyces cerevisiae have diastatic activity. They contain the chimeric gene STA1 that codes for an extracellular glucoamylase which enables the strains to degrade starch and dextrins. Beer contaminations by diastatic yeasts can be dangerous because they can cause super-attenuation due to the consumption of otherwise non-fermentable oligosaccharides, gushing and off-flavours.

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Foot-and-mouth disease (FMD), an economically important disease of livestock, is endemic in Botswana. The country has been affected by this disease since the early 1930s, and FMD virus (FMDV) continues to circulate in both domestic and wild animal populations. Botswana is affected by the Southern African Territories (SAT1-3) of FMDV.

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