[Nephelometry as optic immunochemical method in the laboratory practice].

The authors offer a modification of the nephelometric method for clinical immunology laboratory to be used for measuring proteins (albumin, C3-complement, IgG, IgA, IgM, and alpha 2-macroglobulin) in human biological fluids. The original method making use of only Russian-made reagents and equipment has been used on a full scale for measuring the proteins in the blood serum, cerebrospinal fluid, urine, and saliva of normal subjects. The sensitivity and specificity of the method depend predominantly on the physicochemical parameters of the study.

[Study of lectin-binding segments on the surface of Leishmania gymnoductyli reptulii during in vitro differentiation].

Changes in expression of lectin-binding sites, which are complex carbohydrate structures of Leishmania gymnoductyli reptulii, were studied by the classical lectin agglutination test. The results evidence that this test may be used to assess the level of ontogenesis of Leishmania strains, as well as for the detection and isolation of metacyclic (invasion) stage from Leishmania cell culture by lectins.

Quantitation of Bacillus anthracis by using of soybean agglutinin conjugates.

We examine the possibility of using the soybean agglutinin (SBA) marked by peroxidase (HRP), biotin, FITC, or gold in order to determine the number of Bacillus anthracis cells of vaccine strain STI. It was shown that the technique based on interaction between the lectin and microbial cell walls likely are not inferior in sensitivity to traditional ELISA variants. The sensitivities of methods were 10(4) cells/ml in the case of SBA-biotin, 10(5) cells/ml in the case of SBA-HRP, or 10(6) cells/ml in the cases of SBA-gold and SBA-FITC.

Comparative studies of the interaction between lectins and Leishmania in agglutination tests and enzyme-linked lectin-biotin assays (ELLBA).

It was demonstrated that soybean agglutinin and peanut agglutinin aggregated all the investigated species of Leishmania, including virulent and avirulent members of L. major in agglutination tests. Concanavalin A and Pisum sativum agglutinin were shown to aggregate L.

[Immunoenzyme analysis of antigen-specific determination of HBsAG-containing circulating immune complexes (CIC HBsAG/IgM and CIC HBsAG/IgG) in blood serum of patients with HBV-infection].

A complex enzyme immunoassay (ELISA) has been designed for antigen-specific determination of HBsAg-containing circulating immune complexes (CIC HBsAg/IgM and CIC HBsAg/IgG) in human blood sera in parallel with registration of free HBsAg and specific antibodies to viruses of hepatitis A, B and D. It is shown that effective formation of HBsAg-containing CIC serologically is registered predominantly as a mutually incompatible marker with detection of free HBsAg (in 70-85% of the cases). CIC HBsAg/IgM and CIC HBsAg/IgG may be registered both in parallel and as mutually exclusive markers.