Molecular Dynamics Study of Chitosan Adsorption at a Silica Surface.

Chitosan is a nontoxic biopolymer with many potential biomedical and material applications due to its biodegradability, biocompatibility, and antimicrobial properties. Here, fully atomistic molecular dynamics simulations and enhanced sampling methods have been used to study the adsorption mechanism of chitosan oligomers on a silica surface from an aqueous solution. The free energy of adsorption of chitosan on a silica surface was calculated to be 0.

LevSeq: Rapid Generation of Sequence-Function Data for Directed Evolution and Machine Learning.

Sequence-function data provides valuable information about the protein functional landscape but is rarely obtained during directed evolution campaigns. Here, we present Long-read every variant Sequencing (LevSeq), a pipeline that combines a dual barcoding strategy with nanopore sequencing to rapidly generate sequence-function data for entire protein-coding genes. LevSeq integrates into existing protein engineering workflows and comes with open-source software for data analysis and visualization.

Pediatric orthopedic all-terrain vehicle injury patterns, surgeries, and complications: appreciating the true morbidity and impact.

All-terrain vehicle (ATV) accidents frequently cause orthopedic injuries. Previous studies have reported the frequency of fractures in ATV injuries. No studies have provided detailed assessments of fracture patterns, types of operative intervention, or risks for multiple surgeries.

Covalently Cross-Linked Polyelectrolyte Complex Nanoparticles with Enhanced Stability against Dissociation at High Ionic Strength.

Polyelectrolyte complex nanoparticles (PECNPs) often fully dissociate into individual polycations (PC) and polyanions (PA) at high salinities. Herein, we introduce a novel type of colloidally stable PECNP in which the PC is cross-linked, in this case branched polyethylenimine (PEI) to limit this dissociation, even in solutions up to 5.2 M NaCl or 5.

The Australian clinical trial landscape: Perceptions of rural, regional and remote health service capacity and capability.

Background: Access to clinical trials is limited for rural, regional and remote Australians, adding to the current health inequity between rural and metropolitan populations. The Australasian Teletrial Model was developed to bring clinical trials "closer to home". In 2020, the Australian Teletrial Program was funded to expand and support the uptake of the model across six Australian states and territories.