Publications by authors named "K L Bedi"
Atrial fibrillation (AF) is the most common sustained arrhythmia in humans, yet the molecular basis of AF remains incompletely understood. To determine the cell type-specific transcriptional changes underlying AF, we perform single-nucleus RNA-seq (snRNA-seq) on left atrial (LA) samples from patients with AF and controls. From more than 175,000 nuclei we find that only cardiomyocytes (CMs) and macrophages (MΦs) have a significant number of differentially expressed genes in patients with AF.
View Article and Find Full Text PDFWe performed gene expression profiling of mRNA/cDNA isolated from N = 117 flow sorted CLL. We detected aberrant expression of the metabolic enzyme branched chain amino acid transferase (BCAT1) in CLL with del17p/TP53mut. Through extensive validation, we confirmed the highly preferential expression of BCAT1 in CLL with del17p/TP53mut (66%) or trisomy 12 (77%).
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- Drug shortages, especially for medications like protamine, challenge clinical practices; conventional dosing methods may lead to excess usage and risk of harm.* -
- In a study involving cardiac surgery patients, researchers compared a fixed 250 mg dose of protamine to a ratio-based dosing method (1 mg for every 100 U heparin) for effectiveness in reversing heparin's effects.* -
- Results showed no significant difference in the effectiveness of both dosing methods, but the fixed dose resulted in significantly lower total protamine usage without increasing postoperative bleeding.*
Article Synopsis
- Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) lack critical nanoscale structures called dyads that are vital for heart function, especially during heart failure.
- The reduced expression of the protein cardiomyopathy-associated 5 (CMYA5) leads to disorganization of these dyads in heart failure and disrupts their assembly in hiPSC-CMs.
- A miniaturized version of CMYA5 delivered via adeno-associated virus effectively improved the structure of dyads and normalized heart function under stress, enhancing calcium handling and contractile forces in hiPSC-CMs.