A method for the preparation of purified antigens of coxsackievirus B3 from a large volume of cell culture supernatant.

A simple procedure was used for the concentration and partial purification of coxsackievirus B3 (Nancy strain). For a large-scale production of virus. Vero cells grown in roller bottles were used.

Experimental live tick-borne encephalitis vaccine (Labgat E5 "14" virus clone): volunteers 1 and 2 years after single-dose immunization.

Groups of volunteers given intramuscularly 5 or 6.5 dex or perorally 6.5 dex newborn mouse icLD50 of the plaquesegregated "14" clone of the Langat E5 virus strain (tick-borne encephalitis comples) were followed for periods of 12 and 24-27 months.