Electromagnetic shock wave in nonlinear vacuum: exact solution.

An analytical approach to the theory of electromagnetic waves in nonlinear vacuum is developed. The evolution of the pulse is governed by a system of nonlinear wave vector equations. An exact solution with its own angular momentum in the form of a shock wave is obtained.

Diffraction of femtosecond pulses; nonparaxial regime.

We present a systematic study of linear propagation of ultrashort laser pulses in media with dispersion, dispersionless media, and vacuum. The applied method of amplitude envelopes makes it possible to estimate the limits of the slowly varying amplitude approximation and to describe an amplitude integrodifferential equation governing propagation of optical pulses in the single-cycle regime in solids. The well-known slowly varying amplitude equation and the amplitude equation for the vacuum case are written in dimensionless form.

Electron microscopic data for the presence of post-meiotic gene expression in isolated ram sperm chromatin.

A whole mount electron microscopic technique facilitated a direct visualization of transcripts in ram spermatozoa in run-on experiments. The localization of transcripts in sperm chromatin by spreading enabled identification of regions where transcription complexes, presumably pre-mRNA species, were seen related to chromatin. In another series of experiments the localization of polymerase II was demonstrated using a specific antibody against the conservative tail domain of the polymerase II molecule, followed by protein A-gold visualization on spread chromatin and on thin sections from mature spermatozoa.

Electron microscopic demonstration of transcription of ram sperm chromatin.

Transcription of ram sperm chromatin was examined by two electron microscopic techniques, namely spreading and thin sections. Labelling by Streptavidin-gold particles permits identification of transcription complexes, that have previously incorporated biotinylated uridine triphosphate. This supports previous electron microscopic data for randomly distributed transcription complexes and the presence of polymerase II molecules, documented by means of specific antibody using immunoelectron microscopy.