A simple LC-MS/MS assay for the quantification of E6011, a novel anti-fractalkine monoclonal antibody, in cynomolgus monkey serum - comparison with ligand binding assay.

E6011 is a monoclonal antibody that is currently under development for the treatment of rheumatoid arthritis. While ligand binding assays (LBAs) are typically employed for the determination of therapeutic antibodies, ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) represents an alternative platform. E6011 in monkey serum was treated with ammonium sulfate to obtain pellets for subsequent processing.

Comparison of active learning algorithms in classifying head computed tomography reports using bidirectional encoder representations from transformers.

Purpose: Systems equipped with natural language (NLP) processing can reduce missed radiological findings by physicians, but the annotation costs are burden in the development. This study aimed to compare the effects of active learning (AL) algorithms in NLP for estimating the significance of head computed tomography (CT) reports using bidirectional encoder representations from transformers (BERT).

Methods: A total of 3728 head CT reports annotated with five categories of importance were used and UTH-BERT was adopted as the pre-trained BERT model.

A novel frameshift mutation in ADCK1 identified in a case of chronic fatigue syndrome successfully treated with oral 5-ALA/SFC.

Chronic Fatigue Syndrome (CFS) is a complex disorder characterized by prolonged, unexplained fatigue and challenging diagnosis. We report the case of a 35-year-old Japanese woman with CFS who had experienced chronic fatigue since the age of 11 years. Despite treatment with modafinil, methylphenidate, levocarnitine, and ubiquinone, the symptoms persisted.

Influences of the short-term dietary carbohydrate restriction on the chicken cecal tonsil.

This study aims to clarify influences of the short-term carbohydrate (CHO) restriction on plasma cells in chicken cecal tonsil (CT) using immunohistochemical and morphometrical techniques. Healthy male layer chickens were randomly divided into the control and three experimental groups. The three experimental groups were fed with diets containing 50%, 25%, and 0% CHO of that in the control diet, respectively.

Maintenance of stem cell self-renewal by sex chromosomal zinc-finger transcription factors.

In this Editorial review, we would like to focus on a very recent discovery showing the global autosomal gene regulation by Y- and inactivated X-chromosomal transcription factors, zinc finger gene on the Y chromosome (ZFY) and zinc finger protein X-linked (ZFX). ZFX and ZFY are both zinc-finger proteins that encode general transcription factors abundant in hematopoietic and embryonic stem cells. Although both proteins are homologs, interestingly, the regulation of self-renewal by these transcriptional factors is almost exclusive to ZFX.