Publications by authors named "K Kazuki"
The development of antibody drugs through animal immunization typically requires the humanization of host antibodies to address concerns about immunogenicity in humans. However, employing an animal model capable of producing human antibodies presents the opportunity to develop antibody drugs without the need for humanization. Despite the ratio of human immunoglobulin (Ig) κ to Igλ usage being approximately 60%:40%, the majority of approved antibody therapeutics are kappa antibodies, and the development of lambda antibodies as therapeutic agents has lagged behind.
View Article and Find Full Text PDFUlcerative colitis (UC) is an incurable intestinal disease, with current treatments mainly focused on inflammation control and, in severe cases, surgical resection. Recent studies have highlighted the need for new therapies that promote tissue regeneration. R-spondin-1 (RSPO1) and interleukin-22 (IL-22) have shown anti-inflammatory and regenerative effects in UC models, but have short half-lives and poor targeting abilities.
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- Microcell-mediated chromosome transfer (MMCT) is a new technique for manipulating chromosome numbers using human induced pluripotent stem cells (hiPSCs) to create disease models with hyperaneuploidy.
- The study highlights the efficiency of using hiPSCs as both donor and recipient cells in the chromosome transfer process, allowing for the tagging and transfer of specific chromosomes like Chr21 and ChrY using CRISPR-Cas9.
- This innovative method aims to improve the study of rare diseases and enhance our understanding of early development by integrating key chromosomal regions into hiPSCs.
Article Synopsis
- A new technology was developed to extend the lifespan and maintain the differentiation ability of human mesenchymal stem cells (hMSCs) using nonintegrative, temperature-sensitive Sendai virus vectors.
- This "rejuvenation" process allows for improved cell growth and cloning abilities while keeping the cells' normal characteristics and functionality.
- The method ensures that modified cells can be supplied for research and clinical applications without altering their genetic makeup, potentially benefiting drug development and cell therapy.
Cancer-related lymphedema represents a potential complication of cancer treatment. The aim of this study is to evaluate the effectiveness of the combination of lymphatico-venular anastomosis and liposuction in the treatment of secondary lymphedema. We present a retrospective analysis of patients affected by cancer-related unilateral limb lymphedema.
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