strains from Western Ghats of India possess nematocidal property against larvae of goats.

Nematocidal properties of spore crystal mixtures of six (Bt) strains (KAU 49, 50, 52, 61, 99 and 424) collected from Western Ghats, a biodiversity hot spot of India, were analysed against larvae isolated from goats. One dose nematocidal assay dose response to lyophilised spore-crystal mixtures (SCM) of the six Bt strains were determined by adding 200 μg/mL of each SCMs to culture plate wells containing aqueous suspension of larvae. Out of the strains screened, KAU 50 and 424 were found to possess nematocidal properties.

Crystal Protein of a Novel Bacillus thuringiensis Strain Inducing Cell Cycle Arrest and Apoptotic Cell Death in Human Leukemic Cells.

Parasporal inclusions of a native non haemolytic Bacillus thuringiensis strain KAU 59 was screened for its cytotoxicity against human lymphocytic leukemic cell line jurkat and normal human lymphocytes. The cytotoxicity of proteinase activated and non activated solubilised parasporal inclusions against both cell lines was assessed by Cell Titer 96 Aqueous Non Radioactive Cell Proliferation Assay Kit using MTS. The 50 per cent effective concentration (EC) values were deduced from log probit analysis at 48 h.