Enhanced fetal hemoglobin production via dual-beneficial mutation editing of the HBG promoter in hematopoietic stem and progenitor cells for β-hemoglobinopathies.

Background: Sickle cell disease (SCD) and β-thalassemia patients with elevated gamma globin (HBG1/G2) levels exhibit mild or no symptoms. To recapitulate this natural phenomenon, the most coveted gene therapy approach is to edit the regulatory sequences of HBG1/G2 to reactivate them. By editing more than one regulatory sequence in the HBG promoter, the production of fetal hemoglobin (HbF) can be significantly increased.

Factors associated with interstitial lung disease and the progressive fibrosing phenotype in rheumatoid arthritis-related interstitial lung disease.

Background: The risk factors for interstitial lung disease (ILD) in rheumatoid arthritis (RA) are inconsistent among previous studies. Furthermore, the factors associated with the emergence of the recently defined progressive fibrosing (PF) phenotype are unknown. Herein, we analyze the risk factors for ILD in RA.

Augmenting cybersecurity through attention based stacked autoencoder with optimization algorithm for detection and mitigation of attacks on IoT assisted networks.

The Internet of Things (IoT) network is a fast-growing technology, which is efficiently used in various applications. In an IoT network, the massive amount of connecting nodes is the existence of day-to-day communication challenges. The platform of IoT uses a cloud service as a backend for processing information and maintaining remote control.

Carboxymethyl hexanoyl chitosan drop-coated simple disposable paper electrochemical sensor for quality monitoring of vanillin.

The food and pharmaceutical sectors frequently utilize vanillin (VAN), a food ingredient with a pleasing flavor and aroma. However, excessive consumption of VAN causes several health problems, including liver and kidney damage, headaches, skin conditions, nausea, and vomiting. To prevent health problems, it is crucial to identify and control the amount of VAN in food and drugs.