Monoclonal antibodies to a tumour-associated antigen isolated from circulating immune complexes of bovine leukemia virus-infected cattle.

From circulating immune complexes (ICs) of BLV-infected cattle, an antigen preparation was produced that contained some virus-specific proteins and a tumour-associated antigen. Eleven hybridoma clones were produced that secreted monoclonal antibodies (MoAbs) to this tumour-associated antigen, and two of them, MoAbs 1B4 and 1E9, were used in further studies. Most antibodies were of IgG1 subclass and showed no cytotoxic activity towards lymphocytes of BLV-positive cattle or to certain tumour cells.

Antigen-antibody complexes in experimental infection with Trichinella spiralis and their role in the development of kidney lesions.

The kidneys of rats, experimentally infected with Trichinella spiralis were studied by means of light and fluorescent microscopy. The results obtained suggest that this is a case of a proliferative intra- and extracapillary nephritis. Highest intensity of the pathological process was noted between the 30th and 50th day of the infection.

Antigammaglobulin antibodies in experimental adjuvant arthritis.

Several-fold administration of complete Freund's adjuvant in rat provoked acute arthritis accompanied by production of antigammaglobulin antibodies in a high percentage from the animals studied; their sera precipitate homologous and heterologous aggregated IgG and demonstrate hemagglutination titers up to 1 : 1792. To confirm the Ig type of the studied antibodies, rat sera were fractionated by gel filtration on Sephadex G200 by pH = 4.0 and by preparative ultra-centrifugation by pH = 3.