Magea13 attenuates myocardial injury in acute myocardial infarction by inhibiting the cAMP-PKA signaling pathway.

Object: Acute myocardial infarction (AMI) is a serious cardiovascular disease for which there are still no effective therapeutic options available, and melanoma-associated antigen-A13 (Magea13), a member of the MAGE superfamily, has an unknown role in AMI. This study aims to investigate the potential role and molecular mechanisms of Magea13 in myocardial injury associated with AMI through in vivo and in vitro experiments.

Methods: Firstly, differentially expressed genes (DEGs) and signaling pathways were screened by RNA sequencing.

[The HMGB1, caspase-1 and gasdermin D-mediated pyroptosis in high-altitude retinopathy: an experimental study].

To investigate the role of high mobility group protein B1 (HMGB1), cysteine aspartic protease 1 (caspase-1) and gasdermin D in the pathogenesis of high altitude retinopathy (HAR). This study is an experimental research. Twelve 8- to 10-week-old male c57BL/6J mice without a specific pathogen grade were randomly divided into the HAR group (HAR model) and control group (normal pressure and oxygen environment) according to the random number table method.

Application of scRNA-seq in Dental Research: Seeking Regenerative Clues From the Structure of Tooth and Periodontium in Physical or Pathological States.

This review presents a comprehensive overview of single-cell RNA sequencing (scRNA-seq) analyses used to study tooth and periodontal tissues. The intricate cellular composition of both teeth and periodontium are revealed, leading to the identification of new cell types and tracing lineage profiles for each cell type. Herein, we summarize the progression of dental and periodontal tissue formation, tooth homeostasis, and regenerative mechanisms.

Colorectal cancer hot spot mutations attenuate the ASXL-MLL4 interaction.

Human additional sex combs like (ASXL) proteins are involved in the maintenance of both transcriptional activation and repression through their ability to bind multiple chromatin regulators, including two tumor suppressors: deubiquitinase BAP1 and methyltransferase MLL4 (KMT2D). The ASXL genes are often altered in colorectal cancer (CRC), and ASXL1 is one of the four hub genes related to the pathogenesis of CRC. Here, we show that MLL4 and BAP1 interdependently target specific genomic regions and positively or negatively regulate expression of a subset of genes in the human colon carcinoma HCT116 cells.

A dual-reactivity-based surface-enhanced Raman spectroscopy nanosensor for the simultaneous imaging of hypochlorite and nitric oxide in living cells.

A surface-enhanced Raman spectroscopy (SERS) nanosensor with dual-reactivity is developed for the simultaneous imaging of hypochlorite (ClO) and nitric oxide (NO) in living cells. Utilizing the specific reactions between functional molecules and ClO and NO, respectively, the 2-mercapto-4-methoxy-phenol (2-MP) and o-phenylenediamine (OPD) molecules are synchronously assembled on the surface of gold nanoparticles to fabricate the dual-function nanosensors. The advantages of SERS technology, narrow peaks for spectral multiplexing and fingerprint information, further facilitate the simultaneous detection of ClO and NO.