Hepatic pyruvate carboxylase expression differed prior to hyperketonemia onset in transition dairy cows.

Fatty acids (FA) provide an energy source to the liver during negative energy balance; however, when FA influx is excessive, FA can be stored as liver lipids or incompletely oxidized to β-hydroxybutyrate (BHB). The objectives of this study were to quantify plasma and liver FA profiles and hepatic gene expression in cows diagnosed with hyperketonemia (HYK; BHB ≥ 1.2 mM) or not (nonHYK; BHB < 1.

Short communication: Effect of manipulating fatty acid profile on gluconeogenic gene expression in bovine primary hepatocytes.

During the peripartum period, dairy cows experience both an increase in circulating fatty acid (FA) profile and a change in circulating FA profile, which have been shown to alter regulation of gluconeogenic genes. The objective was to quantify gene expression of key enzymes involved in gluconeogenesis and FA transport into the mitochondria in primary hepatocytes in response to exposure to an FA mixture mimicking what is circulating in a transition dairy cow with or without enrichment of C16:0, C18:0, and C18:1. Primary hepatocytes were isolated from 4 Holstein bull calves 3 d of age (± standard deviation 2 d) and cultured.

Feeding high oleic acid soybeans in place of conventional soybeans increases milk fat concentration.

It is well established in the literature that feeding free vegetable oils rich in oleic acid results in greater milk fat secretion than does feeding linoleic-rich oils. The objectives of these experiments were to analyze the effects of oleic and linoleic acid when fed in the form of full-fat soybeans and the interaction between soybean particle size and fatty acid (FA) profile. Soybeans were included in diets on an iso-ether extract basis and diets were balanced for crude protein using soybean meal.

Mining data from milk infrared spectroscopy to improve feed intake predictions in lactating dairy cows.

Feed intake is one of the most important components of feed efficiency in dairy systems. However, it is a difficult trait to measure in commercial operations for individual cows. Milk spectrum from mid-infrared spectroscopy has been previously used to predict milk traits, and could be an alternative to predict dry matter intake (DMI).

The effects of adding fat to diets of lactating dairy cows on total-tract neutral detergent fiber digestibility: A meta-analysis.

The objective of this meta-analysis was to determine the effects of supplemental fat on fiber digestibility in lactating dairy cattle. Published papers that evaluated the effects of adding fat to the diets of lactating dairy cattle on total-tract neutral detergent fiber digestibility (ttNDFd) and dry matter intake (DMI) were compiled. The final data set included 108 fat-supplemented treatment means, not including low-fat controls, from 38 publications.