Labeling of methyl groups: a streamlined protocol and guidance for the selection of H precursors based on molecular weight.

A streamlined one-day protocol is described to produce isotopically methyl-labeled protein with high levels of deuterium for NMR studies. Using this protocol, the DO and H-glucose content of the media and protonation level of ILV labeling precursors (ketobutyrate and ketovalerate) were varied. The relaxation rate of the multiple-quantum (MQ) state that is present during the HMQC-TROSY pulse sequence was measured for different labeling schemes and this rate was used to predict upper limits of molecular weights for various labeling schemes.

Does loneliness before the age of twelve indirectly affect impaired control over drinking, alcohol use, and problems through perceived stress?

Loneliness is the pain of feeling socially isolated from others (Russell et al., 1980). The Stress-Dampening Hypothesis (Marlatt, 1987; Sayette, 1993; Sher, 1987) posits that individuals drink to alleviate negative affect.

Self-Perception of Transition Readiness Amongst Pediatric Patients with Childhood Heart Disease.

This study assesses self-report of transition readiness among adolescents and young adults (ages 12-25 years) with childhood and congenital heart disease (CHD), receiving care at Hasbro Children's Hospital, whose CHD diagnosis warranted transfer to adult cardiology care. Patients were mailed the American Academy of Pediatrics/American College of Physicians Transition Readiness for Youth Assessment survey. Confidence scores ranged between 0 (not) and 10 (very).

Why do children struggle on analogical reasoning tasks? Considering the role of problem format by measuring visual attention.

Given the importance of analogical reasoning to bootstrapping children's understanding of the world, why is this ability so challenging for children? Two common sources of error have been implicated: 1) children's inability to prioritize relational information during initial problem solving; 2) children's inability to disengage from salient distractors. Here, we use eye tracking to examine children and adults' looking patterns when solving scene analogies, finding that children and adults attended differently to distractors, and that this attention predicted performance. These results provide the most direct evidence to date that feature based distraction is an important way children and adults differ during early analogical reasoning.

Developmental differences in memory reactivation relate to encoding and inference in the human brain.

Despite the fact that children can draw on their memories to make novel inferences, it is unknown whether they do so through the same neural mechanisms as adults. We measured memory reinstatement as participants aged 7-30 years learned new, related information. While adults brought memories to mind throughout learning, adolescents did so only transiently, and children not at all.