A system for in vitro selection of fully 2'-modified RNA aptamers.

SFM4-3, KOD DGLNK, and Therminator polymerase are investigated for their compatibility with SELection with Modified Aptamers (SELMA), an aptamer discovery method that enables incorporation of large nucleobase modifications such as glycans. We demonstrated that with suitable modifications to the primer design and protocol, these enzymes are compatible with SELMA, enabling 2'-fluoro or 2'-methoxy ribose modifications at all positions. In the case of 2'-fluoro modifications, Therminator exhibits cleaner incorporation of an alkyne-modified nucleobase for click chemistry.

Revitalising strong cultural connections and resilience: Co-designing a pilot Elder-led mentorship program for Indigenous mothers in a remote northern community in Alberta, Canada.

Connecting with traditional knowledge and culture promotes the well-being of Indigenous parents and creates healthy environments for child development. Community Elders in a remote northern community in Alberta, Canada, collaborated with researchers to design a pilot Elders Mentoring Program. The programme aims to support young Indigenous mothers(-to-be), bringing back cultural traditions and teachings.

Development of a Diagnostic IgM Antibody Capture ELISA for Detection of Anti-Cache Valley Virus Human IgM.

Cache Valley virus (CVV), a mosquito-borne orthobunyavirus, causes epizootics in ruminants characterized by congenital malformations and fetal death in North America. Only seven human infections have been identified; limited information exists on its potential as a human teratogen. Diagnosis of CVV infections relies on the plaque reduction neutralization test (PRNT), which requires live virus, is time-consuming, and cannot differentiate between recent and past infections.

Enhancing radiation therapy student's cultural competency and safety of Canadian Indigenous populations using cultural immersion.

Introduction: Indigenous peoples in Canada have a higher cancer burden and shorter life expectancy compared to the non-Indigenous population. Canada's colonial legacy has resulted in many Indigenous people experiencing high mistrust in healthcare providers which can result in healthcare avoidance, such as delays seeking preventative care as well as lower screening rates. This may be compounded by language barriers as well as a lack of culturally safe care in healthcare settings.