Cleavage polyembryos as explants for plant regeneration in wheat.

The increased efficiency of embryogenic callus formation and regeneration of plantlets was the result of using immature induced polyembryos of wheat rather than single embryos as primary explants. Plant regeneration was effected via embryogenesis, organogenesis, and a combined process beginning as embryogenesis and proceeding as organogenesis.

Somatic embryogenesis from cultured leaf segments of Zea mays.

Basal leaf segments of 3 to 4 week old maize (Zea mays L.) seedlings plated on SH medium with 30 μM dicamba produced embryogenic callus and/or somatic embryos. Histological evidence showed that some of the embryos arose directly from the explant.

The genetics and developmental regulation of L-glycerol 3-phosphate dehydrogenase.

In addition to reviewing the genetic regulation of L-glycerol 3-phosphate dehydrogenase during development in the mouse, new evidence is presented that the electrophoretic properties of L-glucerol 3-phosphate dehydrogenase in Mus castaneus are determined by an allele (d) at the Gdc-1 locus. Accordingly there are three alleles at the Gdc-1 locus; the b allele in C57BL/6J mice differs from the d allele in electrophoretic properties and the c allele in BALB/cJ mice differs from the d allele with respect to both heat denaturation and electrophoretic properties. Identical segregation patterns of the L-glycerol 3-phosphate dehydrogenase phenotypes in liver, kidney, and skeletal muscle from offspring of an F2 generation produced from parents with the c/d genotype suggest that the Gdc-1 locus is the major structural locus for L-glycerol 3-phosphate dehydrogenase in these tissues.