Publications by authors named "K Ino"
Spherical biosamples such as immunobeads, cells, and cell aggregates have been widely used in bioapplications. The bioactivity of individual spherical biosamples in highly sensitive assays and individual analyses must be evaluated in a high-throughput manner. Electrochemiluminescence (ECL) imaging was recently proposed for the high-throughput analysis of diffusive molecules from spherical biosamples.
View Article and Find Full Text PDFUnderstanding the interactions between lipid membranes and peptides is crucial for controlling bacterial and viral infections, and developing effective drugs. In this study, we proposed the use of electrochemiluminescence (ECL) microscopy in a solution of [Ru(bpy)] and tri--propylamine to monitor alterations in the lipid membranes due to peptide action. A planar artificial lipid membrane served as a model platform, and its surface was observed using ECL microscopy during exposure to melittin, a representative membrane lytic peptide.
View Article and Find Full Text PDFBackground And Objectives: A pharmacokinetic model has been developed to quantify the drug-drug interactions of tacrolimus with concentration-dependent inhibition of cytochrome P450 (CYP) 3A4 from voriconazole and clarithromycin based on the CYP3A5 and CYP2C19 genotypes.
Methods: This retrospective study recruited unrelated bone marrow transplant recipients receiving oral tacrolimus concomitantly with voriconazole and clarithromycin. The published population pharmacokinetic model that implemented genotypes of CYP3A5 (tacrolimus) and CYP2C19 (voriconazole) was integrated.
Article Synopsis
- The study investigates the prevalence and factors associated with red blood cell (RBC) alloimmunization in transfused patients, focusing on how clinical factors relate to the development and disappearance of RBC antibodies.
- Data from 1194 patients across five institutions showed that 4.6% developed new antibodies after transfusion, with the number of RBC units transfused significantly influencing this outcome.
- The research suggests that antibodies identified through specific testing methods may often fade away quickly and emphasizes the importance of thorough antibody screening, particularly for patients receiving many transfusions, to avoid missing potentially relevant antibody information.
Article Synopsis
- This study introduces a new method for creating two-layer microfluidic devices with porous electrodes, which are valuable for bioanalytical applications, particularly in microphysiological systems.
- The innovative fabrication process involves 3D printing sacrificial layers using Pluronic F-127 ink, placing a porous gold electrode in between, and then removing the sacrificial layers to create the device structure.
- The newly developed device was capable of performing electrochemical assays to measure serotonin levels, demonstrating its potential for future use in various biological applications.