Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods.

Background: Exosomes are emerging targets for biomedical research. However, suitable methods for the isolation of blood plasma-derived exosomes without impurities have not yet been described.

Aim: Therefore, we investigated the efficiency and purity of exosomes isolated with potentially suitable methods; differential ultracentrifugation (UC) and size exclusion chromatography (SEC).

[Side effects of oral contraceptives in the first treatment cycle].

In order to compare the secondary effects of oral contraceptive pills during the first cycle of application, 308 women between the age of 25 and 45 were interrogated by questionnaires about their subjective complaints. The patients received a contraceptive within an In-vitro-Fertilization-program (IVF-program) in preparation for a stimulation treatment to follow. The study consisted of two parts: at the beginning a pilot study was carried out in which retrospectively a pill containing 0.

[Radioactive cesium 137 and cesium 134 in follicle and seminal fluid].

Radioactive caesium nuclids were measured in follicular fluid and seminal plasma after the nuclear power station accident of Chernobyl. Immediately after the event (June - Aug. 86) both Cs 137 and Cs 134 were negative, with the exception of one sample.