Substitution of leucine by glutamate perturbs VopE localization to mitochondria: Lessons from yeast model system.

VopE, a type III effector protein of Vibrio cholerae, modulates host mitochondrial function. Mitochondrial entry of VopE is directly linked with an N-terminal precursor sequence known as the mitochondrial targeting sequence or MTS. MTS of VopE is constituted with 23 amino acids.

Modulation of gut microbiome in response to the combination of Nissle 1917 and sugars: a pilot study using host-free system reflecting impact on interpersonal microbiome.

Introduction: The differential effects of probiotic, prebiotic, and synbiotic formulations on human health are dictated by the inter-individual gut microbial profile. The effects of probiotics such as Nissle 1917 (ECN) on gut microbiota may vary according to the microbiome profiles of individuals and may be influenced by the presence of certain carbohydrates, which can impact microbial community structure and treatment results.

Method: Processed fecal samples from donors having contrasting lifestyles, dietary patterns, and disease histories were mixed with 5 × 10 CFU/mL ECN with or without 1% (w/v) sugars (glucose, galactose, or rice starch) in a host-free system.

Recurrent hybridization and gene flow shaped Norway and Siberian spruce evolutionary history over multiple glacial cycles.

Most tree species underwent cycles of contraction and expansion during the Quaternary. These cycles led to an ancient and complex genetic structure that has since been affected by extensive gene flow and by strong local adaptation. The extent to which hybridization played a role in this multi-layered genetic structure is important to be investigated.