Publications by authors named "K Havlickova"
The quantification of cellular metabolic activity via MTT assay has become a widespread practice in eukaryotic cell studies and is progressively extending to bacterial cell investigations. This study pioneers the application of MTT assay to evaluate the metabolic activity of biofilm-forming cells within bacterial biofilms on nanofibrous materials. The biofilm formation of Staphylococcus aureus and Escherichia coli on nanomaterials electrospun from polycaprolactone (PCL), polylactic acid (PLA), and polyamide (PA) was examined.
View Article and Find Full Text PDFTo develop microbiologically safe nanofibrous materials, it is crucial to understand their interactions with microbial cells. Current research indicates that the morphology of nanofibers, particularly the diameter of the fibers, may play a significant role in biofilm formation and retention. However, it has not yet been determined how the fiber diameter of poly-ε-caprolactone (PCL), one of the most widely used biopolymers, affects these microbial interactions.
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- The study investigates how different sterilization methods and solvent systems affect the degradation of electrospun biodegradable nanofibers made from polyesters, particularly for medical uses like vascular bandaging.
- Findings show that the sterilization method, either ethylene oxide or gamma irradiation, significantly influences how the materials degrade, with distinct degradation patterns observed depending on the method used.
- The choice of solvent for preparing the polymer solution also plays a crucial role, with the most accelerated degradation occurring in PLCL fibers made from organic acids and sterilized through gamma irradiation.
Oxidative stress, microparticles, and E-selectin do not depend on HIV suppression.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub
February 2024
Article Synopsis
- The study explored oxidative stress and inflammation in people with HIV undergoing antiretroviral treatment to see if these factors contribute to early aging diseases.
- Researchers measured oxidative stress through malondialdehyde levels and inflammation using E-selectin levels in 213 HIV-positive individuals compared to healthy controls.
- Results showed that HIV patients had significantly higher levels of oxidative stress and inflammation, independent of their viral load and immune status, suggesting these may play a role in premature aging.
Skin wound healing is a complex physiological process that involves various cell types, growth factors, cytokines, and other bioactive compounds. In this study, a novel dual-function multilayered nanofibrous membrane is developed for chronic wound application. The membrane is composed of five alternating layers of polycaprolactone (PCL) and poly (vinyl alcohol) (PVA) nanofibers (PCL-PVA) with a dual function: the PCL nanofibrous layers allow cell adhesion and growth, and the PVA layers enriched with incorporated platelet lysate (PCL-PVA + PL) serve as a drug delivery system for continuous release of bioactive compounds from PL into an aqueous environment.
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