Differential Cytotoxic Effects of Cell-Free Supernatants of Emerging Pathogens and on Four Cell Lines Reveal Vero Cells as a Putative Candidate for Cytotoxicity Analysis.

and are recognized as emerging pathogens with zoonotic potential. Despite their increasing importance, there is a paucity of data on the cytotoxicity of these two pathogens. Therefore, in the present study, we investigated the cytotoxic potentials of the cell-free supernatants from 10 and 15 isolates for their cytotoxic effects on four different cell lines (CHO, Vero, HeLa, and MDCK).

of porcine origin exhibits an open pan-genome within a single clonal complex: insights from comparative genomic analysis.

Introduction: Although spp., including , have emerged as important zoonotic foodborne pathogens globally, the understanding of the genomic epidemiology of of porcine origin is limited.

Methods: As pigs are an important reservoir of , we analyzed genomes that were isolated ( = 3) from pigs and sequenced (this study) them along with all other genomes for which pig intestines, pig feces, and pigs were mentioned as sources in the NCBI database up to January 6, 2023.

Power and area efficient FIR filter using Radix- 2 multiplier for de-noise the electrooculography (EOG) signal.

This article introduces a novel FIR filter using a Radix-2r multiplier combined with 4:2 and 3:2 compressors for denoising Electrooculography (EOG) signals. This approach replaces traditional ripple-carry adders with compressors to add the partial products generated by the Radix-2r multiplier, resulting in reduced delay and energy consumption. The FIR filter is implemented in gate-level Verilog HDL, verified using ModelSim and Altera DSP Builder, and synthesized with the Cadence RTL compiler.

Cell type-specific impact of aging and Alzheimer disease on hippocampal CA1 perforant path input.

The perforant path (PP) carries direct inputs from entorhinal cortex to CA1 pyramidal neurons (PNs), with an impact dependent on PN position across transverse (CA1a-CA1c) and radial (superficial/deep) axes. It remains unclear how aging and Alzheimer disease (AD) affect PP input, despite its critical role in memory and early AD. Applying recordings and two-photon microscopy in slices from mice up to 30 months old, we interrogated PP responses across PN subpopulations and compared them to Schaffer collateral and intrinsic excitability changes.