Publications by authors named "K H M Prange"
Background And Hypothesis: Kidney macrophage infiltration is a histological hallmark of vasculitic lesions and is strongly linked to disease activity in anti-neutrophil cytoplasmic antibodies (ANCA)-associated glomerulonephritis (AGN). The precise mechanisms by which kidney macrophages influence local inflammation and long-term damage remain largely unknown.
Methods: Here, we investigate kidney macrophage diversity using single-cell transcriptome analysis of 25 485 freshly retrieved unfrozen, high-quality kidney CD45+ immune cells from five AGN patients during active disease, a lupus nephritis and nephrectomy control.
Article Synopsis
- Epigenetic age acceleration (EAA) in atherosclerotic plaques is linked to future cardiovascular events, showing that older plaque age can predict mortality risk similar to overall epigenetic age estimators.
- In a study involving 485 human carotid plaques, EAA was correlated with clinical indicators, with patients showing higher EAA having conditions like diabetes and obesity.
- Single-cell RNA sequencing identified smooth muscle and endothelial cells as key contributors to plaque EAA, with the endothelial-to-mesenchymal transition process being linked to accelerated aging in those cells.
Background: Oxidized phospholipids play a key role in the atherogenic potential of lipoprotein(a) (Lp[a]); however, Lp(a) is a complex particle that warrants research into additional proinflammatory mediators. We hypothesized that additional Lp(a)-associated lipids contribute to the atherogenicity of Lp(a).
Methods: Untargeted lipidomics was performed on plasma and isolated lipoprotein fractions.
Article Synopsis
- Histopathological studies of atherosclerotic plaques reveal that diverse lesion types necessitate improved classification methods to understand their clinical significance.
- An analysis of gene expression in 654 human carotid plaques identified five main plaque types, each linked to specific clinical outcomes and differences in cell composition.
- Findings suggest that a particular plaque type with severe symptoms is associated with inflammatory and fibrotic cells, and ongoing research is exploring potential biomarkers for distinguishing these plaque phenotypes.
Article Synopsis
- * The study utilized RNA sequencing data to create gene regulatory networks, revealing two main SMC phenotypes in women: a vulnerable myofibroblast-like network and a contractile network, which differed in expression levels compared to males.
- * Findings suggest that female atherosclerosis involves specific gene networks that promote plaque vulnerability, with important implications for understanding disease progression and potential treatment strategies.