A new fast-neutron source for radiobiological research.

A biomedical cyclotron facility primarily dedicated to radionuclide production has been extended by the addition of an experimental fast-neutron source for radiobiological and biophysical studies. Several beams of fast-neutrons with different average energies and LET distributions can now be provided. The neutrons are produced by bombarding berryllium targets with 18-32 McV protons or 8.

[Separation of urological tumor cells by means of a cell saver combined with a membrane filter. A new technique for autotransfusion?].

Three long duration cancer cell lines were marked with radioactive methionine S and mixed with a defined quantity of concentrated red cells. The mixture was separated according to a standardized method with cell saver and two membrane filters. The marked cancer cells were counted with a liquid scintillation cell counter.

[Separation of urologic tumors cells from Cell Saver blood using a membrane filter. A new method in autotransfusion?].

Tumor cells of three urological longterm cell lines have been labelled with 35[S]-Methionin and added to red cell concentrates. Red cell concentrates rich in tumor cells were passed through a cell saver and two special membranfilters under standard conditions. The 35[S]-Methionin labelled tumor cells were detected by liquid scintillation counting.

Response of the rat Dunning R3327-AT1 prostate tumor to treatment with fractionated fast neutrons.

Reports indicate that cancer of the prostate, soft tissue sarcomas, salivary gland tumors, and melanomas respond well to fast-neutron treatment. To better understand the action of fast neutrons on such tumor tissues, we have begun studies with the versatile Dunning rat prostate tumor system. In our initial studies with the R3327-AT1 subline we observed a relative biological effectiveness (RBE) of approximately 3 for single doses of 14-meV fast neutrons.