Publications by authors named "K H Brackett"
The Endoplasmic Reticulum (ER)-resident HSP70 chaperone BiP (HSPA5) plays a crucial role in maintaining and restoring protein folding homeostasis in the ER. BiP's function is often dysregulated in cancer and virus-infected cells, conferring pro-oncogenic and pro-viral advantages. We explored BiP's functions during infection by the Kaposi's sarcoma-associated herpesvirus (KSHV), an oncogenic gamma-herpesvirus associated with cancers of immunocompromised patients.
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- Outbred laboratory mice (Mus musculus) are popular in biomedical research due to their high reproduction rates and availability, and high throughput genome sequencing (HTS) helps ensure the genetic quality and reproducibility of studies using these mice.
- A study on a newly established Swiss-derived outbred population (J:ARC) utilized exome sequencing to explore their genetic architecture and found characteristics typical of well-maintained outbred stocks, comparing it to the Diversity Outbred (J:DO) stock.
- The J:DO stock showed greater genetic variation and interindividual variability but had a higher frequency of potentially damaging genetic variants, indicating that diverse genetic backgrounds can help buffer against harmful mutations under varying selective pressures.
Uncovering viral gene functions requires the modulation of gene expression through overexpression or loss-of-function. CRISPR interference (CRISPRi), a modification of the CRISPR-Cas9 gene editing technology, allows specific and efficient transcriptional silencing without genetic ablation. CRISPRi has been used to silence eukaryotic and prokaryotic genes at the single-gene and genome-wide levels.
View Article and Find Full Text PDFHox genes pattern the anterior-posterior axis of animals and are posited to drive animal body plan evolution, yet their precise role in evolution has been difficult to determine. Here, we identified evolutionary modifications in the Hox gene Abd-B that dramatically altered its expression along the body plan of Drosophila santomea. Abd-B is required for pigmentation in Drosophila yakuba, the sister species of D.
View Article and Find Full Text PDFPediatric feeding difficulties are complex and multifactorial in nature. Children who need assessment and individualized treatment for complex feeding problems are best served by an interdisciplinary treatment team. The medical, motor, and behavioral approach to treating pediatric feeding problems is presented as an avenue to treatment.
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