Publications by authors named "K Gruden"
Article Synopsis
- Plants face attacks from pathogens that use effectors like necrosis- and ethylene-inducing peptide-1-like proteins (NLPs) to invade and damage them.
- NLPs, known for causing cell death and tissue damage, disrupt the plant's plasma membrane through unique mechanisms that create small, temporary membrane ruptures.
- Recent research utilized confocal fluorescence microscopy to analyze how NLP interacts with model plant cell membranes, revealing that NLP's permeabilization effects depend on its concentration and time of exposure, and confirming its binding and structural changes on these membranes.
Article Synopsis
- Ethylene response factor StPti5 in potatoes is identified as a susceptibility factor that negatively impacts immunity against pathogens like potato virus Y and Ralstonia solanacearum, which operate through different mechanisms.
- The study reveals that StPti5 is broken down in healthy plants through autophagy but accumulates in the nucleus during infection, indicating its role in the plant's defense response.
- It also shows that StPti5 is regulated by the interaction of ethylene and salicylic acid pathways, influencing other ERFs and downregulating the ubiquitin-proteasome pathway, contributing to understanding plant immunity regulation.
Potato () is the most popular tuber crop and a model organism. A variety of gene models for potato exist, and despite frequent updates, they are not unified. This hinders the comparison of gene models across versions, limits the ability to reuse experimental data without significant re-analysis, and leads to missing or wrongly annotated genes.
View Article and Find Full Text PDFHigh Throughput Image-Based Phenotyping for Determining Morphological and Physiological Responses to Single and Combined Stresses in Potato.
J Vis Exp
June 2024
Article Synopsis
- - High throughput image-based phenotyping allows researchers to analyze plant development and performance non-invasively, capturing traits like biomass and photosynthetic efficiency using various imaging sensors.
- - In a study on potato plants (cv. Lady Rosetta), researchers tested responses to different stress treatments (control, drought, heat, waterlogging, and combinations) to understand how plants react to overlapping environmental challenges.
- - Results indicated that waterlogging was the most harmful stress, causing rapid physiological changes, while all stresses negatively affected growth and photosynthesis, highlighting the importance of this technology for studying plant resilience to climate change.
Protein complexes from edible oyster mushrooms (Pleurotus sp.) composed of pleurotolysin A2 (PlyA2) and pleurotolysin B (PlyB) exert toxicity in feeding tests against Colorado potato beetle (CPB) larvae, acting through the interaction with insect-specific membrane sphingolipid. Here we present a new strategy for crop protection, based on in planta production of PlyA2/PlyB protein complexes, and we exemplify this strategy in construction of transgenic potato plants of cv Désirée.
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