Publications by authors named "K Gevaert"
Limited proteolysis coupled to mass spectrometry (LiP-MS) has emerged as a powerful proteomic tool for studying protein conformations. Since its introduction in 2014, LiP-MS has expanded its scope to explore complex biological systems and shed light on disease mechanisms, and has been used for protein drug research. This review discusses the evolution of the technique, recent technical advances, including enhanced protocols and integration of machine learning, and diverse applications across various experimental models.
View Article and Find Full Text PDFPlants continuously respond to changing environmental conditions to prevent damage and maintain optimal performance. To regulate gas exchange with the environment and to control abiotic stress relief, plants have pores in their leaf epidermis, called stomata. Multiple environmental signals affect the opening and closing of these stomata.
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- Olduvai protein domains, linked to the NBPF gene family, show significant expansion in humans and correlate with brain size and neuron numbers in primates, as well as human brain variations like microcephaly and macrocephaly.
- Research indicates that overexpression of the Olduvai gene may lead to downregulation of mitochondrial functions, particularly affecting the electron transport chain and NADH dehydrogenase activity, based on transcriptome and proteome analyses.
- The findings suggest that this downregulation could slow development processes in primates, especially humans, potentially resulting in an extended neurogenic period that allows for the production of more neurons and a larger brain size.
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- Proteogenomics integrates genomics and mass spectrometry to uncover both standard and alternative proteins, enhancing personalized medicine for conditions like ovarian cancer.
- Through RNA-sequencing and protein database generation, researchers identified 128 reference proteins and 30 alternative proteins unique to ovarian cancer cell lines, uncovering a specific mutation in an alternative protein.
- Advanced techniques like cross-linking mass spectrometry revealed interactions between alternative and reference proteins, suggesting that some alternative proteins may play crucial roles in important cellular processes such as DNA replication and repair.
To survive extreme desiccation, seeds enter a period of quiescence that can last millennia. Seed quiescence involves the accumulation of protective storage proteins and lipids through unknown adjustments in protein homeostasis (proteostasis). Here, we show that mutation of all six type-II metacaspase (MCA-II) proteases in Arabidopsis thaliana disturbs proteostasis in seeds.
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