Publications by authors named "K Galloway"
A synthetic protein-based winner-take-all neural network controls cell fate decisions.
View Article and Find Full Text PDFArticle Synopsis
- Understanding the roles of RNA and proteins is crucial for determining cell fate and designing effective gene circuits.
- The study utilizes HCR Flow-FISH to measure mRNA and protein levels in single cells, revealing that stronger synthetic promoters lead to higher RNA transcripts and translation rates.
- The research highlights the importance of polyadenylation signals and coding sequences, which can significantly affect mRNA and protein profiles, providing a new framework for comparing native and synthetic gene regulation.
Unlabelled: While transcription factors (TFs) provide essential cues for directing and redirecting cell fate, TFs alone are insufficient to drive cells to adopt alternative fates. Rather, transcription factors rely on receptive cell states to induce novel identities. Cell state emerges from and is shaped by cellular history and the activity of diverse processes.
View Article and Find Full Text PDFArticle Synopsis
- The U.S. Air Force relies on remotely piloted aircraft (RPA) aircrew and support personnel for critical military missions, which can lead to increased stress and psychological health risks.
- To counter these issues, a “combat-to-reconstitution” policy was trialed, aimed at giving RPA personnel more downtime for recovery.
- A study involving 397 RPA personnel gathered mixed feedback on the policy's implementation and highlighted that factors like work overload contributed to stress and potential burnout, pointing to areas for improvement.
Targeting DNA payloads into human (h)iPSCs involves multiple time-consuming, inefficient steps that must be repeated for each construct. Here, we present STRAIGHT-IN Dual, which enables simultaneous, allele-specific, single-copy integration of two DNA payloads with 100% efficiency within one week. Notably, STRAIGHT-IN Dual leverages the STRAIGHT-IN platform to allow near-scarless cargo integration, facilitating the recycling of components for subsequent cellular modifications.
View Article and Find Full Text PDF