Rapid identification of Fusobacterium nucleatum and Fusobacterium necrophorum by fluorescence in situ hybridization.

Identification of clinically relevant Fusobacterium spp. is hampered by their slow growth, their frequent occurrence in polymicrobial culture, and the low reliability of biochemical differentiation methods. A newly developed fluorescence in situ hybridization (FISH) assay allowed reliable and rapid identification of Fusobacterium necrophorum and Fusobacterium nucleatum from culture.

Therapeutic efficacy of moxifloxacin in a murine model of severe systemic mixed infection with Escherichia coli and Bacteroides fragilis.

The therapeutic efficacy of moxifloxacin was studied in an experimental murine model of a systemic aerobic/anaerobic mixed infection and compared to therapies with either imipenem or ciprofloxacin plus metronidazole. Groups of 20 mice each were intravenously (iv) infected with approximately 2.5 x 10(6) colony forming units (CFU) of Escherichia coli and 2 x 10(7)CFU of Bacteroides fragilis.

[The pathogen spectrum of acute bacterial rhinitis/sinusitis and antibiotic resistance].

Background And Objective: Bacterial rhinosinusitis is one of the most frequent indications for an antibiotic therapy. The objective of this study was the analysis of the current pathogen spectrum and its antimicrobial susceptibility.

Material And Methods: Between 1999 and 2004, 188 specimens obtained from 170 patients with acute, purulent rhinosinusitis were analysed.

Meningitis due to Haemophilus influenzae type f in an 8-year-old girl with congenital humoral immunodeficiency.

We report on an 8-year-old Haemophilus influenzae type b-vaccinated girl with meningitis due to H. influenzae type f. The girl had an oro-facio-digital syndrome and a hypogammaglobulinemia, obviously predisposing the patient to invasive infections.